2D et 3D imaging, live samples, images analysis
The C3M imaging facility provide helps , training, technological and biological developpement for cell and tissue imaging to scientists comming from the plubilc or private companies.
Microscopy network Imagerie Côte d’Azur
The C3M microscopy platform is labeled Infrastructure in Biology, Health and Agronomy (IBiSA). It is a member of the Microscopy Imaging Côte d'Azur (MICA) network which pools the technical platforms (light and electronic microscopy, cytometry, image analysis) of 8 partners in academic research on the Côte d'Azur.
Imaging manager Marie Irondelle
I train you on the devices of the platform.
I can help you develop protocols.
I answer your questions about the operation or the technical possibilities of the platform.
marie.irondelle@univ-cotedazur.fr
Imaging Engineer Anne Doye
I can help you find technical solutions and train you on the platform's devices.
anne.doye@univ-cotedazur.fr
To access the platform, users must have been trained by platform staff and sign the user charter and one training sheet per device. Users must register for each device on the C3M intranet. The platform can provide specific imaging media (multi-well ibidi, fluorodish, etc.) or antibodies needed when implementing new protocols.
As stipulated in the charter, publications containing data generated on the platform must mention it in the acknowledgments.
The Incucyte is a microscopy device placed in an incubator. It allows to follow living cells in a stable and controlled environment and to automatically acquire images over long periods (days, weeks). Acquisitions are possible in white light and in "green" and "red" fluorescence, which allows multifactorial screening conditions and the analysis of different factors (confluence, cell number, cell death, wound healing....).
Specifications techniques
This microscope is equipped with a Nikon DS-L3 color camera and is dedicated to the visualization of histological sections stained with different contrast methods (carmine red, hematoxylin/eosin, Masson's trichrome...).
Specifications techniques
This upright open-field microscope from Leica is equipped with an ORCA-ER camera (Hamamatsu) and allows routine fluorescence imaging.
Specifications techniques
Confocal microscopy offers several advantages over conventional optical microscopy, including shallow depth of field, elimination of out-of-focus blurry, and the ability to collect serial optical sections from thick specimens.
This microscope allows the acquisition of thick samples such as tissues, spheroids or organoids, as well as the acquisition of small intracellular organelles such as endosomes, the Golgi or the mitochondrial network.
| Inserm | Académique (hors Inserm) | Privé | |
|---|---|---|---|
| Use of the devices (per heure - 1h minimum) | 38,84€ | 51,73€ | 80€ |
