Analysis of cellular oxygen consumption, glycolysis, ATP and mitochondrial metabolism
The Cellular Metabolism platform has several pieces of equipment: a Seahorse, a YSI bioanlayzer, an OmniLog and hypoxia chambers to study the metabolic functions of cells (oxygen consumption, glycolysis, ATP production...). This equipment was obtained with the financial support of the Conseil Départemental des Alpes-Maritimes, the Canceropôle PACA and the European Research Council.
This platform is home to Met'Connect: the PACA Cancéropôle's new Structuring Action for Tumour Metabolism Analysis.
Manager of the Seahorse and Hypoxia chambers Nathalie Mazure
Do not hesitate to contact me if you have any questions.
nathalie.mazure@univ-cotedazur.fr
Manager of the YSI Johanna Chiche
I can help you find technical solutions or train you on YSI.
johanna.chiche@univ-cotedazur.fr
Manager of Met'Connect and OmniLog Constance Nau
I can help you find technical solutions and train you on OmniLog.
constance.nau@inserm.fr
To access the platform, users must be trained by the staff in charge of the equipment. Users must book each device on the C3M intranet. The platform shares the purchase of the Seahorse plates (available from the person in charge of the Imaging Platform, Marie Irondelle).
We have two hypoxia chambers that allow us to cultivate cells at variable oxygen concentrations.
The Seahorse is an instrument that measures in real time oxygen consumption (OCR) and the pH of the culture medium. It is used to analyze the energy metabolism of cells. It is used with specific 96-well plates available from Marie Irondelle who is in charge of the Imaging Platform.
More info
The YSI is an automated system for measuring glucose, lactate, glutamine and glutamate in the culture medium.
More info
OmniLog (Biolog) has been funded by the Conseil Départemental des Alpes-Maritimes and the Cancéropôle PACA. It can be used to establish the metabolic phenotype of a biological sample (cells, organoids, tissue explants). It is possible to analyse the 'metabolic preference' of cells in relation to more than 1,400 metabolites, hormones and cytokines, or their sensitivity to inhibitors in 96-well plates. It is an ideal technology for screening molecules and characterising cell metabolism.
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